Open Access

Comparison of methods to examine the endogenous peptides of fetal calf serum

  • Declan Williams1,
  • Peihong Zhu1,
  • Peter Bowden1,
  • Catherine Stacey2,
  • Mike McDonell2,
  • Paul Kowalski2,
  • Jane Marie Kowalski2,
  • Ken Evans3,
  • Eleftherios P. Diamandis3, 4,
  • K. W. Michael Siu3 and
  • John Marshall1, 3Email author
Clinical Proteomics2:20167

https://doi.org/10.1385/CP:2:1:67

Abstract

There is a great desire to relate the patterns of endogenous peptides in blood to human disease and drug response. The best practices for the preparation of blood fluids for analysis are not clear and also relatively few of the peptides in blood have been identified by tandem mass spectrometry. We compared a number of sample preparation methods to extract endogenous peptides including C18 reversed phase, precipitation, and ultrafiltration. We examined the results of these sample preparation methods by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and liquid chromatography-tandem mass spectrometry (MS/MS) using MALDI-TOF/TOF and electrospray ionization-ion trap. Peptides from solid-phase extraction with C18 in the range of hundreds of femtomoles per spot were detected from the equivalent of 1 μL of serum by MALDI-TOF. We observed endogenous serum peptides from fibrinogen α- and β-chain, complement C3, α-2-HS-glycoprotein, albumin, serine (or cysteine) proteinase inhibitor, factor VIII, plasminogen, immunoglobulin, and other abundant blood proteins. However, we also recorded significant MS/MS spectra from tumor necrosis factor-α-, major histocompatibility complex-, and angiotensin-related peptides, as well as peptides from collagens and other low-abundance proteins. Amino acid substitutions were detected and a phosphorylated peptide was also observed. This is the first time the endogenous peptides of fetal serum have been examined by MS and where peptides from low-abundance proteins, phosphopeptides, and amino acid substitutions were detected.

Key words

Fetal calf serum liquid chromatography-electrospray ionization mass spectrometry matrix-assisted laser desorption/ionization time-of-flight MALDI ESI tandem mass spectrometry

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