O’Farrell PH: High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975, 250: 4007-4021.
PubMed Central
PubMed
Google Scholar
Magdeldin S, Zhang Y, Bo X, Yoshida Y, Yamamoto T: Biochemistry, Genetics and Molecular Biology “Gel Electrophoresis - Principles and Basics”. 2012, Rijeka, Croatia: INTECH,
Google Scholar
Anderson NG, Matheson A, Anderson NL: Back to the future: the human protein index (HPI) and the agenda for post-proteomic biology. Proteomics. 2001, 1: 3-12.
Article
CAS
PubMed
Google Scholar
Magdeldin S, Li H, Yoshida Y, Enany S, Zhang Y, Xu B, Fujinaka H, Yaoita E, Yamamoto T: Comparison of two dimensional electrophoresis mouse colon proteomes before and after knocking out aquaporin 8. J Proteomics. 2010, 73: 2031-2040.
Article
CAS
PubMed
Google Scholar
Jungblut PR, Seifert R: Analysis by high-resolution two-dimensional electrophoresis of differentiation-dependent alterations in cytosolic protein pattern of HL-60 leukemic cells. J Biochem Biophys Methods. 1990, 21: 47-58.
Article
CAS
PubMed
Google Scholar
Wu W, Tang X, Hu W, Lotan R, Hong WK, Mao L: Identification and validation of metastasis-associated proteins in head and neck cancer cell lines by two-dimensional electrophoresis and mass spectrometry. Clin Exp Metastasis. 2002, 19: 319-326.
Article
CAS
PubMed
Google Scholar
Enany S, Yoshida Y, Magdeldin S, Bo X, Zhang Y, Enany M, Yamamoto T: Two dimensional electrophoresis of the exo-proteome produced from community acquired methicillin resistant Staphylococcus aureus belonging to clonal complex 80. Microbiol Res. 2013, 168: 504-511.
Article
CAS
PubMed
Google Scholar
Rabilloud T, Chevallet M, Luche S, Lelong C: Two-dimensional gel electrophoresis in proteomics: past, present and future. J Proteomics. 2010, 73: 2064-2077.
Article
CAS
PubMed
Google Scholar
Bech-Serra JJ, Borthwick A, Colome N, ProteoRed C, Albar JP, Wells M, Sanchez del Pino M, Canals F: A multi-laboratory study assessing reproducibility of a 2D-DIGE differential proteomic experiment. J Biomol Tech. 2009, 20: 293-296.
PubMed Central
PubMed
Google Scholar
Bland AM, Janech MG, Almeida JS, Arthur JM: Sources of variability among replicate samples separated by two-dimensional gel electrophoresis. J Biomol Tech. 2010, 21: 3-8.
PubMed Central
PubMed
Google Scholar
Chevalier F: Highlights on the capacities of “Gel-based” proteomics. Proteome Sci. 2010, 8: 23-
Article
PubMed Central
PubMed
Google Scholar
Chevallet M, Luche S, Diemer H, Strub JM, Van Dorsselaer A, Rabilloud T: Sweet silver: a formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry. Proteomics. 2008, 8: 4853-4861.
Article
CAS
PubMed
Google Scholar
Shevchenko A, Wilm M, Vorm O, Mann M: Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem. 1996, 68: 850-858.
Article
CAS
PubMed
Google Scholar
Pinol-Roma S, Choi YD, Matunis MJ, Dreyfuss G: Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins. Genes Dev. 1988, 2: 215-227.
Article
CAS
PubMed
Google Scholar
Kjaersgard IV, Jessen F: Two-dimensional gel electrophoresis detection of protein oxidation in fresh and tainted rainbow trout muscle. J Agric Food Chem. 2004, 52: 7101-7107.
Article
PubMed
Google Scholar
Gorg A, Postel W, Domscheit A, Gunther S: Two-dimensional electrophoresis with immobilized pH gradients of leaf proteins from barley (Hordeum vulgare): method, reproducibility and genetic aspects. Electrophoresis. 1988, 9: 681-692.
Article
CAS
PubMed
Google Scholar
Gorg A, Postel W, Gunther S: The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis. 1988, 9: 531-546.
Article
CAS
PubMed
Google Scholar
Wilkins MR, Gasteiger E, Sanchez JC, Bairoch A, Hochstrasser DF: Two-dimensional gel electrophoresis for proteome projects: the effects of protein hydrophobicity and copy number. Electrophoresis. 1998, 19: 1501-1505.
Article
CAS
PubMed
Google Scholar
Corthals GL, Wasinger VC, Hochstrasser DF, Sanchez JC: The dynamic range of protein expression: a challenge for proteomic research. Electrophoresis. 2000, 21: 1104-1115.
Article
CAS
PubMed
Google Scholar
Santoni V, Rabilloud T, Doumas P, Rouquie D, Mansion M, Kieffer S, Garin J, Rossignol M: Towards the recovery of hydrophobic proteins on two-dimensional electrophoresis gels. Electrophoresis. 1999, 20: 705-711.
Article
CAS
PubMed
Google Scholar
Zahedi RP, Meisinger C, Sickmann A: Two-dimensional benzyldimethyl-n-hexadecylammonium chloride/SDS-PAGE for membrane proteomics. Proteomics. 2005, 5: 3581-3588.
Article
CAS
PubMed
Google Scholar
Babu GJ, Wheeler D, Alzate O, Periasamy M: Solubilization of membrane proteins for two-dimensional gel electrophoresis: identification of sarcoplasmic reticulum membrane proteins. Anal Biochem. 2004, 325: 121-125.
Article
CAS
PubMed
Google Scholar
Pasquali C, Fialka I, Huber LA: Preparative two-dimensional gel electrophoresis of membrane proteins. Electrophoresis. 1997, 18: 2573-2581.
Article
CAS
PubMed
Google Scholar
Rabilloud T, Adessi C, Giraudel A, Lunardi J: Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis. 1997, 18: 307-316.
Article
CAS
PubMed
Google Scholar
Falvo S, Di Carli M, Desiderio A, Benvenuto E, Moglia A, America T, Lanteri S, Acquadro A: 2-D DIGE analysis of UV-C radiation-responsive proteins in globe artichoke leaves. Proteomics. 2011, 12 (3): 448-60.
Article
Google Scholar
Semaan SM, Sang Q-XA: Prefractionation enhances loading capacity and identification of basic proteins from human breast cancer tissues. Anal Biochem. 2011, 411: 8-
Article
Google Scholar
Stasyk T, Huber LA: Zooming in: fractionation strategies in proteomics. Proteomics. 2004, 4: 3704-3716.
Article
CAS
PubMed
Google Scholar
Boisvert FM, Lam YW, Lamont D, Lamond AI: A quantitative proteomics analysis of subcellular proteome localization and changes induced by DNA damage. Mol Cell Proteomics. 2010, 9: 457-470.
Article
PubMed Central
CAS
PubMed
Google Scholar
Cascio S, Zhang L, Finn OJ: MUC1 protein expression in tumor cells regulates transcription of proinflammatory cytokines by forming a complex with nuclear factor-kappaB p65 and binding to cytokine promoters: importance of extracellular domain. J Biol Chem. 2011, 286: 42248-42256.
Article
PubMed Central
CAS
PubMed
Google Scholar
Molloy MP, Herbert BR, Walsh BJ, Tyler MI, Traini M, Sanchez JC, Hochstrasser DF, Williams KL, Gooley AA: Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis. 1998, 19: 837-844.
Article
CAS
PubMed
Google Scholar
Zhu K, Yan F, O’Neil KA, Hamler R, Lubman DM, Lin L, Barder TJ: Proteomic analysis using 2-D liquid separations of intact proteins from whole-cell lysates. Curr Protoc Protein Sci. 2004, Chapter 23: Unit 23 23-
Google Scholar
Zhang Y, Yoshida Y, Nameta M, Xu B, Taguchi I, Ikeda T, Fujinaka H, Mohamed SM, Tsukaguchi H, Harita Y, Yaoita E, Yamamoto T: Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney. Nephrol Dial Transplant. 2010, 25: 1785-1795.
Article
CAS
PubMed
Google Scholar
Karlsson K, Cairns N, Lubec G, Fountoulakis M: Enrichment of human brain proteins by heparin chromatography. Electrophoresis. 1999, 20: 2970-2976.
Article
CAS
PubMed
Google Scholar
Fountoulakis M, Takacs MF, Berndt P, Langen H, Takacs B: Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography. Electrophoresis. 1999, 20: 2181-2195.
Article
CAS
PubMed
Google Scholar
Schagger H, von Jagow G: Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem. 1991, 199: 223-231.
Article
CAS
PubMed
Google Scholar
Kofoed EM, Vance RE: Blue native polyacrylamide gel electrophoresis to monitor inflammasome assembly and composition. Methods Mol Biol. 2013, 1040: 169-183.
Article
CAS
PubMed
Google Scholar
Wang F, Wang L, Xu Z, Liang G: Identification and analysis of multi-protein complexes in placenta. PloS one. 2013, 8: e62988-
Article
PubMed Central
CAS
PubMed
Google Scholar
Salzano AM, Novi G, Arioli S, Corona S, Mora D, Scaloni A: Mono-dimensional blue native-PAGE and bi-dimensional blue native/urea-PAGE or/SDS-PAGE combined with nLC-ESI-LIT-MS/MS unveil membrane protein heteromeric and homomeric complexes in Streptococcus thermophilus. J Proteomics. 2013, 94: 240-261.
Article
CAS
PubMed
Google Scholar
Halligan BD, Ruotti V, Jin W, Laffoon S, Twigger SN, Dratz EA: ProMoST (Protein Modification Screening Tool): a web-based tool for mapping protein modifications on two-dimensional gels. Nucleic Acids Res. 2004, 32: W638-644.
Article
PubMed Central
CAS
PubMed
Google Scholar
Hiller K, Schobert M, Hundertmark C, Jahn D, Munch R: JVirGel: calculation of virtual two-dimensional protein gels. Nucleic Acids Res. 2003, 31: 3862-3865.
Article
PubMed Central
CAS
PubMed
Google Scholar
Farley AR, Link AJ: Identification and quantification of protein posttranslational modifications. Methods Enzymol. 2009, 463: 725-763.
Article
CAS
PubMed
Google Scholar
Bjellqvist B, Ek K, Righetti PG, Gianazza E, Gorg A, Westermeier R, Postel W: Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications. J Biochem Biophys Methods. 1982, 6: 317-339.
Article
CAS
PubMed
Google Scholar
Gorg A, Weser J, Westermeier R, Postel W, Weidinger S, Patutschnick W, Cleve H: Isoelectric focusing with immobilized pH gradients for the analysis of transferrin (Tf) subtypes and variants. Hum Genet. 1983, 64: 222-226.
Article
CAS
PubMed
Google Scholar
Lopez MF: Nonequilibrium pH gel electrophoresis (NEPHGE). Methods Mol Biol. 1999, 112: 129-131.
CAS
PubMed
Google Scholar
Slibinskas R, Razanskas R, Zinkeviciute R, Ciplys E: Comparison of first dimension IPG and NEPHGE techniques in two-dimensional gel electrophoresis experiment with cytosolic unfolded protein response in Saccharomyces cerevisiae. Proteome Sci. 2013, 11: 36-
Article
PubMed Central
CAS
PubMed
Google Scholar
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685.
Article
CAS
PubMed
Google Scholar
Rozalska M, Szewczyk EM: Staphylococcus cohnii hemolysins - isolation, purification and properties. Folia Microbiol (Praha). 2008, 53: 521-526.
Article
CAS
Google Scholar
Hoogland C, Sanchez JC, Tonella L, Binz PA, Bairoch A, Hochstrasser DF, Appel RD: The 1999 SWISS-2DPAGE database update. Nucleic Acids Res. 1999, 2000 (28): 286-288.
Google Scholar