An integrated proteomic and glycoproteomic approach uncovers differences in glycosylation occupancy from benign and malignant epithelial ovarian tumors
- Qing Kay Li†1, 2Email authorView ORCID ID profile,
- Punit Shah†1,
- Yuan Tian1,
- Yingwei Hu1,
- Richard B. S. Roden1,
- Hui Zhang1 and
- Daniel W. Chan1
© The Author(s) 2017
Received: 19 January 2017
Accepted: 24 April 2017
Published: 10 May 2017
Epithelial ovarian carcinomas encompass a heterogeneous group of diseases with a poor 5-year survival rate. Serous carcinoma is the most common type. Most FDA-approved serum tumor markers are glycoproteins. These glycoproteins on cell surface or shed into the bloodstream could serve as therapeutic targets as well as surrogates of tumor. In addition to glycoprotein expressions, the analysis of protein glycosylation occupancy could be important for the understanding of cancer biology as well as the identification of potential glycoprotein changes in cancer. In this study, we used an integrated proteomics and glycoproteomics approach to analyze global glycoprotein abundance and glycosylation occupancy for proteins from high-grade ovarian serous carcinoma (HGSC) and serous cystadenoma, a benign epithelial ovarian tumor, by using LC–MS/MS-based technique.
Fresh-frozen ovarian HGSC tissues and benign serous cystadenoma cases were quantitatively analyzed using isobaric tags for relative and absolute quantitation for both global and glycoproteomic analyses by two dimensional fractionation followed by LC–MS/MS analysis using a Orbitrap Velos mass spectrometer.
Proteins and N-linked glycosite-containing peptides were identified and quantified using the integrated global proteomic and glycoproteomic approach. Among the identified N-linked glycosite-containing peptides, the relative abundances of glycosite-containing peptide and the glycoprotein levels were compared using glycoproteomic and proteomic data. The glycosite-containing peptides with unique changes in glycosylation occupancies rather than the protein expression levels were identified.
In this study, we presented an integrated proteomics and glycoproteomics approach to identify changes of glycoproteins in protein expression and glycosylation occupancy in HGSC and serous cystadenoma and determined the changes of glycosylation occupancy that are associated with malignant and benign tumor tissues. Specific changes in glycoprotein expression or glycosylation occupancy have the potential to be used in the discrimination between benign and malignant epithelial ovarian tumors and to improve our understanding of ovarian cancer biology.
Ovarian cancer is the fifth most common cancer in women in the US, with 22,440 new cases diagnosed and more than 14,080 cancer-related deaths in 2017 . Approximately 80% of patients presented with locally advanced and/or metastatic disease at the time of diagnosis and only ~40% survive five years, although early stage disease can be treated successfully [1, 2]. The poor outcome of patients has not changed much in the past three decades, despite advances in surgery and chemotherapies and major efforts to develop a screening test with greater predictive value than a standalone serum test for the tumor-shed glycoprotein CA-125, which is FDA approved for monitoring therapy. The current largest and most promising clinical screening trial, the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS), which includes 202,638 women over 14-year period, has suggested for the first time that a multimodal screening approach with repeat serum CA-125 testing and transvaginal ultrasonography could potentially reduce the mortality; however, it is still not yet conclusive [3–5]. There remains a need to find better methods that can be use in screening for ovarian cancer while it is still curable with conventional therapy.
Ovarian carcinoma is a heterogeneous disease with various histological subtypes, making the development of a single biomarker challenging and suggesting focus on the most impactful subtype is warranted [6–12]. Most ovarian cancers are epithelial in origin, with high grade serous carcinomas accounting for 70–80% of cases and the rarer subtypes, including clear cell (3%), endometrioid (<5%), mucinous (<3%) carcinoma and others [2, 8–12]. Each histological subtype is now being considered separate disease with characteristic cytogenetic features, molecular signatures, oncogenic signaling pathways and clinical/biological behaviors [8–12]. However, based on clinical characteristics, histological patterns and molecular signatures, ovarian cancer can be divided into two types—type I, which comprises endometrioid, clear cell, mucinous, and low-grade serous cancer, and type II, which comprises high-grade serous carcinoma (HGSC), undifferentiated carcinomas and carcinosarcoma [2, 8–12].
Type I ovarian carcinomas are considered to arise via a well-defined adenoma-carcinoma sequence from a benign precursor lesion, to evolve into a malignant lesion with a stepwise fashion [2, 8–12]. Type I carcinomas are usually slow growing neoplasms with indolent clinical behavior. They frequently exhibit molecular abnormalities in several genes, such as somatic mutations of CTNNB1 (Catenin (Cadherin-Associated Protein), Beta 1), PTEN (phosphatase and tensin homolog) and PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit α) in endometrioid carcinomas; KRAS (Kirsten rat sarcoma viral oncogene homolog) mutations in mucinous carcinomas; PIK3CA activating and ARID1A inactivating mutations in clear cell carcinomas, and others. By contrast, type II carcinomas originate from the fimbriated end of the fallopian tube and possibly ovarian surface epithelia, develop rapidly and behave aggressively [8–12]. Mutations in TP53 (>90%) are common in type II carcinomas [8–12]. Although it is well-known that different histomorphological ovarian carcinomas correlate with characteristic molecular features and clinical behavior, protein and glycosylation signatures of ovarian carcinoma are still not well-studied.
At cellular level, the majority of proteins either secreted from cells or located on extracellular surface are glycoproteins. Glycoproteins play important roles in the regulation of cellular functions, including cell differentiation, proliferation, cellular interactions with their surrounding environment, and invasion or metastasis of tumor cells . In addition, most FDA approved serum tumor markers are glycoproteins, such as carcinoembryonic antigen (CEA), prostate specific antigen (PSA), CA125, CA19-9 . Therefore, it is important to analyze glycoprotein signatures of ovarian cancer tissue, which may provide critical information for the discovery of tumor-associated proteins. Furthermore, our previous studies have shown that the glycosylation pattern of glycoproteins is also associated with biological characteristics of the cancer [15–17]. Similarly, in ovarian cancer changes of glycoproteins may be a result of changes in protein concentration and/or protein glycosylation occupancy [18–21]. Therefore, it is necessary not only to study the global protein profile, but also the glycoprotein profile in ovarian cancers in the search of novel glycoprotein changes.
Quantitative proteomic analysis of several types of ovarian tumors have demonstrated different protein profiles in ovarian cancers [22–28]. Our recent study of 177 HGSCs has demonstrated characteristic protein profiles in ovarian carcinoma tissues and provided an updated knowledge of the proteomics of ovarian cancer . This large scale of study also uncovers the characteristic phosphoproteins of the HGSC. However, the understanding of protein signature, particularly the profile of glycoprotein in HGSC, is still suboptimal.
In this study, we investigated the global proteome and glycoproteome profiles associated with HGSC and benign serous cystadenoma using quantitative LC–MS/MS-based global proteomics and glycoproteomics approach, to simultaneously identify and quantify global proteome and glycoproteome by iTRAQ labeling and LC–MS/MS; and compared the relative abundance of glycoproteins as well as the change in relative glycosylation occupancy of that protein in malignant versus benign ovarian tumor tissues.
Collection of ovarian tumor and benign tissues
Fresh-frozen tissues of three HGSC (stage IIIc from white females) and three benign serous cystadenoma (from white females) were included. All tissues were stored at −80 °C until use. All diagnoses were rendered by the American Pathology Medical Board certified surgical pathologists based on the H&E (hematoxylin and eosin) stained sections. This study was approved by the Johns Hopkins Medical Institution Review Board (IRB).
Hydrazide resin (Bio-Rad, Hercules, CA), sodium periodate (Bio-Rad, Hercules, CA), tris (2-carboxyethyl) phosphine (TCEP) (Pierce, Rockford, IL), PNGase F (New England Biolabs, Ipswich, MA), sequencing grade trypsin (Promega, Madison, WI), C18 columns (Waters, Sep-Pak Vac) were used in our experiment. Additional lab supply and chemicals were purchased from Sigma-Aldrich. iTRAQ reagent and mass calibration standards were from AB SCIEX (Foster City, CA); BCA assay kit was from Pierce (Rockford, IL).
Peptide extraction and glycopeptide enrichment
Fractionation by basic reverse liquid chromatography (bRPLC)
Labeled peptides were fractionated offline using bRPLC as previously reported [31, 32]. Briefly, approximately 100 µg of 4-plex iTRAQ labeled tryptic peptides or 10 µg of labeled glycopeptides were separated on a reversed phase Zorbax extend-C-18 column (4.6 × 100 mm column containing 1.8-µm particles) from Agilent Technology (Santa Clara, CA) using an Agilent 1220 Infinity HPLC System. The solvent consisted of 10 mM ammonium formate (pH 10) as mobile phase A and 10 mM ammonium formate and 90% ACN (pH 10) as mobile phase B. The separation gradient was set as follows: 2% B for 10 min, from 2 to 8% B for 5 min, from 8 to 35% B for 85 min, from 35 to 95% B for 5 min, and 95% B for 25 min. Ninety-six fractions were collected across the LC separation and were concatenated into 24 fractions for tryptic peptides and 12 fractions for glycopeptides (Fig. 1). The samples were dried in a Speed-Vac and stored at −80 °C until LC–MS/MS analysis.
Fractionated iTRAQ labeled peptides were analyzed on a LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific Inc., Rockford, IL). 1 µg of peptides per injection was loaded on a C18 column (75 µm ID × 15 cm, C18 5 µm, 100 A), and gradient eluted over 100 min at 300 nL/min into the mass spectrometer. The HPLC mobile phase A and B were 0.2% formic acid in HPLC grade water and 0.2% formic acid in HPLC grade acetonitrile, respectively. The mobile phase B was increased from 5 to 40% in 90 min. LC–MS/MS data was obtained using a data dependent analysis of the top ten precursors and a dynamic exclusion of 30 s.
The acquired LC–MS/MS data was searched against the Homo sapiens taxonomy of the IPI Human database V3.87 using Sequest (search algorithm within Proteome Discoverer, Thermo Scientific version 1.3) with following parameters: two missed cleavages allowed, trypsin as cleavage enzyme, a tolerance of 15 ppm on precursors and 0.06 Daltons on the fragment ions. Modifications allowed include: carbamidomethylation of cysteines sets to static, iTRAQ4plex on N-Terminus sets to static, iTRAQ4plex on lysine sets to variable, oxidation of methionine and deamination of asparagine and glutamine set to variable. Data was also searched against a decoy database and filtered with a 1% false discovery rate (FDR).
Glycopeptides were further filtered for consensus sequence of N-linked glycosylation motif NXS/T. The Relative ratios of abundance of N-glycoprotein between cancer and benign samples were calculated.
Results and discussion
Detection of glycoprotein and glycosylation occupancy changes in the HGSC tissue
We first analyzed the first iTRAQ set to identify the proteins and glycoproteins from malignant and benign ovarian tumor tissues in replicate analyses. The overall analytic strategy is schematically illustrated in Fig. 1. It consists of following steps: (1) recover and iTRAQ labeling of peptides from tumor tissue and benign ovarian lesions, (2) combine iTRAQ labeled peptides, (3) simultaneously analyze global and N-glycopeptides in conjunction with extensive fractionation and high-resolution tandem mass spectrometry, (4) analyze and compare profiles of global protein and glycoproteins between HG serous carcinoma and benign lesions. By using this approach, we were able to identify the changes in global proteins and glycoproteins simultaneously.
The iTRAQ technique was used to allow for quantitation in conjunction with extensive fractionation using bRPLC and high-resolution tandem mass spectrometry to provide in-depth of coverage for peptide and protein identification and quantification. A total of 4817 proteins were identified and quantified (Additional file 1: Table S1). Using glycoproteomic analysis, we identified and quantified 1629 unique N-linked glycosite-containing peptides (Additional file 2: Table S2).
Thirty-six glycosite-containing peptides with threefold increased levels in ovarian tumor comparing to benign tumors using glycoproteomic analysis
Folate receptor alpha
Toll-like receptor 7
Prolyl endopeptidase FAP
Prolyl endopeptidase FAP
Lysosome-associated membrane glycoprotein 3
Prolyl endopeptidase FAP
Folate receptor α
Prostatic acid phosphatase
Nuclear pore membrane glycoprotein 210
CUB domain-containing protein 1
Endoplasmin (94 kDa glucose-regulated protein)
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
CUB domain-containing protein 1
Extracellular sulfatase Sulf-1
Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Golgi apparatus protein 1
Glutamate carboxypeptidase 2
Leucine-rich repeats and immunoglobulin-like domains protein 1
4F2 cell-surface antigen heavy chain
Sortilin isoform X1
Amiloride-sensitive amine oxidase
Cell adhesion molecule 1
NHL repeat-containing protein 3
Unique glycosylation occupancy changes in 10 glycosite-containing peptides
Folate receptor alpha
Golgi apparatus protein 1
Leucine-rich repeats and immunoglobulin-like domains protein 1
Cell adhesion molecule 1
4F2 cell-surface antigen heavy chain
Folate receptor α
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
Semaphorin-4B isoform 1
Proteins and glycosite-containing peptides identified from the second iTRAQ experiment with 2 additional benign and 2 additional HGSC tumors were further analyzed to obtain quantitative proteomic and glycoproteomic data to determine the change in each N-linked glycosite and corresponding changes in glycoprotein level. A total of 5183 proteins and 1853 N-linked glycosite-containing peptides were identified and quantified (Additional file 3: Table S3; Additional file 4: Table S4). From these data, the changes in glycosite-containing peptides and global proteins in additional 2 cases of HGSC and 2 benign tumors were quantified. This allowed the determination of the changes of the glycosylation occupancies at each glycosite from glycoproteins in HGSC and benign cases. Besides the well-known issue of missing data in the two different iTRAQ experiments by tandem mass spectrometry, which resulted in different glycosite and protein identifications, we also observed individual variation in glycosylation occupancies in HGSC and benign cases. A large number of sample size is needed to determine the disease-associated changes in glycosylation occupancies versus individual variation.
Merit of current techniques in the detection of glycoproteins
In the past decade, several highly sensitive proteomic techniques, such as high-content quantitative proteomic using LC–MS/MS, SPEG, and iTRAQ labeling have been developed [29–32]. These techniques have an increased sensitivity and throughput capability of accurate analysis of biological samples. The combination of these techniques has tremendously improved our ability for the detection of low abundance proteins in biological samples.
By using these advanced techniques, several recent publications have shown that different histological type of ovarian cancer are associated with distinct type of protein profiles in both tumor tissue and serum samples, including changes of glycoproteins. Abbott et al.  studied tumor-specific glycan changes between tumor and normal ovarian tissue and identified glycoproteins markers that show tumor-specific glycosylation changes. Shetty et al.  identified 10 N-linked sialylated glycopeptides significantly upregulated in ovarian cancer patients’ serum samples. Kuzmanov et al.  discovered 13 sialoglycopeptides in ovarian cyst fluid and ascites fluid of ovarian cancer patients. Using SELDI-TOF–MS platform, Zhang et al. [33, 34] identified differential expressions of several serum proteins in ovarian cancers; and later they developed a FDA-approved multivariate index assay, OVA1 test, including five biomarkers: apolipoprotein A1, transthyretin, transferrin, β-2 microglobulin, and CA125 . The OVA1 and second generation OVA2 tests are currently used to assist in physicians to triage women with suspected pelvic masses. This is important because surgery on ovarian cancer is associated with improved outcomes if performed by a specialist gynecologic oncologist and this test can be used to help triage patients. However, these tests lack the predictive value for routine screening and the knowledge of molecular protein signature of ovarian cancers, particularly HGSC, is still suboptimal.
The glycoproteome of tumor tissue is complex and consists of both high and low abundance glycoproteins. The detection of low abundance glycoproteins is notoriously difficult, due to the obscuring artifact of high abundance glycoproteins. In this study, we performed the isobaric labeling prior to split the samples for proteome and glycoproteome analyses, therefore, we were able to acquire glycopeptide abundance and correlate them with changes of the parent protein abundance to provide the net differences in glycosylation. There are several advantages of our strategy of focusing on the identification of N-glycoprotein changes in protein expression and glycosylation occupancy using the integrated global proteomics and glycoproteomics approach. First, the combination of iTRAQ labeling with extensive fractionation and high performance reversed phase liquid chromatography and high-resolution tandem mass spectrometry can provide high depth of coverage for peptide and protein identification for global proteomics. Second, the SPEG glycopeptides-capturing technique allows the identification and quantification of glycosite-containing peptides from extracellular tumor-derived or secreted proteins in tumor tissues. Finally, the integrated proteomic and glycoproteomic analyses of iTRAQ labeled prior to the capture of glycosite-containing peptides can relate glycopeptide abundance changes in parent protein abundance to provide the net differences in glycosylation. Our approach allows us to identify unique N-glycoproteins whose changes may not be found at global protein levels but can only be identified by glycan levels. Thus, we not only identified N-glycoprotein profile, but also compared N-glycoprotein profile with global protein profile in tumor tissues. However, the small sample size in our study is a limitation. A larger scale of study is necessary to further validate our findings.
Significance of our findings and further direction
HGSC is characterized by mutations of TP53, extensive gene copy number alterations (CNAs), alterations in homologous recombination (HR) and aberrations in certain pathways such as PI3 Kinase, RAS, Notch, FOXM1, and RB1 signaling/cell cycle control [2, 8–11]. In our first iTRAQ experiment, among 1629 glycosite-containing peptides, 36 glycosite-containing peptides with more than threefold increased were identified, respectively. In addition, 10 glycosite-containing peptides revealed increase in glycosylation levels, but without a corresponding change at protein levels. We found unique changes of glycosylation occupancy in these glycoproteins, in comparison to changes of their protein levels. Our findings indicated that the unique glycoprotein occupancy might be specific to each glycoprotein. Our findings need to be further verified in a larger scale of study.
These glycoproteins have been demonstrated to play different biological role in ovarian cancers. For example, cell adhesion molecule I plays critical roles in tumor progression . Folate receptor (FOLR) has also been detected in 78% of HGSC by others, and it has been related by an increased overall survival in ovarian cancers . Interestingly, we also detected the expression of CD180. CD180 is a related member of the Toll-like receptor family, and its expression has been related to tumor stage and progression . Taken together, our study provides evidence that multiplex analysis of tumor tissues could provide important insight for understanding the molecular protiome signature. Our quantitative proteomic analysis sheds a light on the characteristics of the complex (glycol) proteome of ovarian tumors and provides an evidence of tumor-associated protein in HGSC. Further study is necessary to improve our current knowledge in the fields of ovarian cancer.
Finally, an elevated level of ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (member of CD157 pathway), was identified in our study. Both ADP-ribosyl cyclase and CD157 belongs the ADP-ribosyl cyclase gene family. They play important roles in the regulation of cancer cell invasion and progression [39–43]. Several previous publications indicate that CD157 is overexpressed and functions as an ectoenzyme and receptor in ovarian cancer cells [39–43]. Furthermore, CD157 regulates the interaction among tumor cells and the expression of extracellular matrix proteins. Taken together, our finding is consistent with prior reports that the ADP-ribosyl cyclase/CD157 pathway plays a critical role in the regulation of ovarian cancer cell invasion and progression.
In summary, with this comprehensive study of proteome and glycoproteome of ovarian HGSC and benign tissues, we were able to determine not only changes of protein concentrations in global proteome and glycoproteome, but also changes in relative glycosylation occupancy at specific glycosylation sites of the protein. It is important to recognize that certain proteomic changes can only be demonstrated at glycosylation occupancy levels. These types of changes could be easily missed by studies which might only focus on the quantification determination of total glycoprotein or at glycosite levels. The glycosylation occupancy of cancer cell may play an important role in understanding of the biology of ovarian cancers as well as in serving as potential biomarkers for ovarian cancer patients.
QKL, RBSR, HZ and DWC write and critic the manuscript. HZ and DWC design experiments. PS, YT, YWH and HZ analysis of data. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Availability of supporting data
Data are available upon request.
Ethical approval and consent to participate
This study was approved by the Johns Hopkins Medical Institution Review Board (IRB).
This work is partially supported by Drs. Ji & Li Family Foundation (QKL), Ovarian Cancer Research Fund Alliance (Grant No. 458972) (RBSR), HERA Foundation Ovarian Cancer Outside-the-box (Grant No. OSB1) (RBSR), Seed Grant and Public Health Service funding from the National Institutes of Health/the National Cancer Institute/Early Detection Research Network Grants (NIH/NCI/EDRN) U01CA152813 (HZ), U24CA115102 (DWC), the Clinical Proteomic Tumor Analysis Consortium (CPTAC, U24CA160036) (DWC and HZ), and P50CA09825 tissuecore C for funding the tissue banking.
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- Siegel RL, Miller KD, Jemal A. Cancer statistics, 2017. CA Cancer J Clin. 2017;67(1):7–30.View ArticlePubMedGoogle Scholar
- Kurman RJ, Carcangiu ML, Herrington CS, Young RH, editors. WHO classification of tumours of female reproductive organs. 4th ed. Lyon: IARC Press; 2014.Google Scholar
- Jacobs IJ, Menon U, Ryan A, Gentry-Maharaj A, Burnell M, Kalsi JK, Amso NN, Apostolidou S, Benjamin E, Cruickshank D, Crump DN, Davies SK, Dawnay A, Dobbs S, Fletcher G, Ford J, Godfrey K, Gunu R, Habib M, Hallett R, Herod J, Jenkins H, Karpinskyj C, Leeson S, Lewis SJ, Liston WR, Lopes A, Mould T, Murdoch J, Oram D, Rabideau DJ, Reynolds K, Scott I, Seif MW, Sharma A, Singh N, Taylor J, Warburton F, Widschwendter M, Williamson K, Woolas R, Fallowfield L, McGuire AJ, Campbell S, Parmar M, Skates SJ. Ovarian cancer screening and mortality in the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS): a randomised controlled trial. Lancet. 2016;389(10022):945–56.View ArticleGoogle Scholar
- Nezhat FR, Apostol R, Nezhat C, Pejovic T. New insights in the pathophysiology of ovarian cancer and implications for screening and prevention. Am J Obstet Gynecol. 2015;213(3):262–7.View ArticlePubMedGoogle Scholar
- Sölétormos G, Duffy MJ, Othman Abu Hassan S, Verheijen RH, Tholander B, Bast RC Jr, Gaarenstroom KN, Sturgeon CM, Bonfrer JM, Petersen PH, Troonen H, CarloTorre G, Kanty Kulpa J, Tuxen MK, Molina R. Clinical use of cancer biomarkers in epithelial ovarian cancer: updated guidelines from the european group on tumor markers. Int J Gynecol Cancer. 2016;26:43–51.View ArticlePubMedGoogle Scholar
- Tian Y, Yao Z, Roden R, Zhang H. Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics. 2011;11:4677–87.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang H, Liu T, Zhang Z, Payne SH, Zhang B, McDermott JE, Zhou JY, Petyuk VA, Chen L, Ray D, Sun S, Yang F, Chen L, Wang J, Shah P, Cha SW, Aiyetan P, Woo S, Tian Y, Gritsenko MA, Clauss TR, Choi C, Monroe ME, Thomas S, Nie S, Wu C, Moore RJ, Yu KH, Tabb DL, Fenyö D, Bafna V, Wang Y, Rodriguez H, Boja ES, Hiltke T, Rivers RC, Sokoll L, Zhu H, Shih IEM, Cope L, Pandey A, Zhang B, Snyder MP, Levine DA, Smith RD, Chan DW, Rodland KD, The CPTAC Investigators. Integrated proteogenomic characterization of human high-grade serous ovarian cancer. Cell. 2016;166(3):755–65.View ArticlePubMedGoogle Scholar
- Vang R, Shih IM, Kurman RJ. Ovarian low-grade and high-grade serous carcinoma: pathogenesis, clinicopathologic and molecular biologic features, and diagnostic problems. Adv Anat Pathol. 2009;16:267–82.View ArticlePubMedPubMed CentralGoogle Scholar
- Kurman RJ. The origin and molecular pathogenesis of ovarian high-grade serous caricinoma. Ann Oncol. 2013;24(Suppl 10):x16–21.View ArticlePubMedGoogle Scholar
- Cancer Genome Atlas Research Network. Integrated genomic analyses of ovarian carcinoma. Nature. 2011;474:609–15.View ArticleGoogle Scholar
- Symeonides S, Gourley C. Ovarian cancer molecular stratification and tumor heterogeneity: a necessity and a challenge. Front Oncol. 2015;21(5):229.Google Scholar
- Liu J, Konstantinopoulos PA, Matulonis UA. Genomic testing and precision medicine—what does this mean for gynecologic oncology? Gynecol Oncol. 2016;140(1):3–5.View ArticlePubMedGoogle Scholar
- Drake PM, Cho W, Li B, Prakobphol A, Johansen E, Anderson NL, Regnier FE, Gibson BW, Fisher SJ. Sweetening the pot: adding glycosylation to the biomarker discovery equation. Clin Chem. 2010;56:223–36.View ArticlePubMedGoogle Scholar
- Tian Y, Zhang H. Glycoproteomics and clinical applications. Proteomics Clin Appl. 2010;4:124–32.View ArticlePubMedGoogle Scholar
- Li QK, Chen L, Ao MH, Chiu HC, Zhang Z, Zhang H, Chan DW. Serum fucosylated prostate-specific antigen (PSA) improves the differentiation of aggressive from non-aggressive prostate cancers. Theranostics. 2015;5:267–76.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang X, Chen J, Li QK, Peskoe SB, Zhang B, Choi B, Platz EA, Zhang H. Overexpression of alpha (1,6) fucosyltransferase associated with aggressive prostate cancer. Glycobiology. 2014;24:935–44.View ArticlePubMedPubMed CentralGoogle Scholar
- Shah P, Wang X, Yang W, Toghi Eshghi S, Sun S, Höti UN, Pasay J, Rubin A, Zhang H. Integrated proteomic and glycoproteomic analyses of prostate cancer cells reveals glycoprotein alteration in protein abundance and glycosylation. Mol Cell Proteomics. 2015;14:2753–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Abbott KL, Lim JM, Wells L, Benigno BB, McDonald JF, Pierce M. Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis. Proteomics. 2010;10(3):470–81.View ArticlePubMedPubMed CentralGoogle Scholar
- Shetty V, Hafner J, Shah P, Nickens Z, Philip R. Investigation of ovarian cancer associated sialylation changes in N-linked glycopeptides by quantitative proteomics. Clin Proteomics. 2012;9(1):10.View ArticlePubMedPubMed CentralGoogle Scholar
- Kuzmanov U, Musrap N, Kosanam H, Smith CR, Batruch I, Dimitromanolakis A, Diamandis EP. Glycoproteomic identification of potential glycoprotein biomarkers in ovarian cancer proximal fluids. Clin Chem Lab Med. 2013;51(7):1467–76.View ArticlePubMedGoogle Scholar
- Ruhaak LR, Kim K, Stroble C, Taylor SL, Hong Q, Miyamoto S, Lebrilla CB, Leiserowitz G. Protein-specific differential Glycosylation of Immunoglobulins in serum of Ovarian Cancer Patients. J Proteome Res. 2016;15(3):1002–10.View ArticlePubMedGoogle Scholar
- Akbani R, Ng PK, Werner HM, Shahmoradgoli M, Zhang F, Ju Z, Liu W, Yang JY, Yoshihara K, Li J, Ling S, Seviour EG, Ram PT, Minna JD, Diao L, Tong P, Heymach JV, Hill SM, Dondelinger F, Städler N, Byers LA, Meric-Bernstam F, Weinstein JN, Broom BM, Verhaak RG, Liang H, Mukherjee S, Lu Y, Mills GB. A pan-cancer proteomic perspective on The Cancer Genome Atlas. Nat Commun. 2014;29(5):3887.Google Scholar
- Xu Z, Wu C, Xie F, Slysz GW, Tolic N, Monroe ME, Petyuk VA, Payne SH, Fujimoto GM, Moore RJ, Fillmore TL, Schepmoes AA, Levine DA, Townsend RR, Davies SR, Li S, Ellis M, Boja E, Rivers R, Rodriguez H, Rodland KD, Liu T, Smith RD. Comprehensive quantitative analysis of ovarian and breast cancer tumor peptidomes. J Proteome Res. 2015;14(1):422–33.View ArticlePubMedGoogle Scholar
- Yang H, Lau WB, Lau B, Xuan Y, Zhou S, Zhao L, Luo Z, Lin Q, Ren N, Zhao X, Wei Y. A mass spectrometric insight into the origins of benign gynecological disorders. Mass Spectrom Rev. 2015;9999:1–21.Google Scholar
- Musrap N, Tuccitto A, Karagiannis GS, Saraon P, Batruch I, Diamandis EP. Comparative proteomics of ovarian cancer aggregate formation reveals an increased expression of calcium-activated chloride channel regulator 1 (CLCA1). J Biol Chem. 2015;290(28):17218–27.View ArticlePubMedPubMed CentralGoogle Scholar
- Fata CR, Seeley EH, Desouki MM, Du L, Gwin K, Hanley KZ, Hecht JL, Jarboe EA, Liang SX, Parkash V, Quick CM, Zheng W, Shyr Y, Caprioli RM, Fadare O. Are clear cell carcinomas of the ovary and endometrium phenotypically identical? A proteomic analysis. Hum Pathol. 2015;46(10):1427–36.View ArticlePubMedGoogle Scholar
- Russell MR, Walker MJ, Williamson AJ, Gentry-Maharaj A, Ryan A, Kalsi J, Skates S, D’Amato A, Dive C, Pernemalm M, Humphryes PC, Fourkala EO, Whetton AD, Menon U, Jacobs I, Graham RL, Protein Z. A putative novel biomarker for early detection of ovarian cancer. Int J Cancer. 2016. doi:10.1002/ijc.30020.PubMedPubMed CentralGoogle Scholar
- Tian Y, Tan AC, Sun X, Olson MT, Xie Z, Jinawath N, Chan DW, Shih IEM, Zhang Z, Zhang H. Quantitative proteomic analysis of ovarian cancer cells identified mitochondrial proteins associated with paclitaxel resistance proteomics. Proteomics Clin Appl. 2009;3:1288–95.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang H, Li XJ, Martin DB, Aebersold R. Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nat Biotechnol. 2003;21:660–6.View ArticlePubMedGoogle Scholar
- Tian Y, Zhou Y, Elliott S, Aebersold R, Zhang H. Solid-phase extraction of N-linked glycopeptides. Nat Protoc. 2007;2:334–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Yang F, Shen Y, Camp DG 2nd, Smith RD. High-pH reversed-phase chromatography with fraction concatenation for 2D proteomic analysis. Expert Rev Proteomics. 2012;9:129–34.View ArticlePubMedPubMed CentralGoogle Scholar
- Makarov A, Scigelova M. Coupling liquid chromatography to Orbitrap mass spectrometry. J Chromatogr A. 2010;1217(25):3938–45.View ArticlePubMedGoogle Scholar
- Rai AJ, Zhang Z, Rosenzweig J, Shih I, Pham T, Fung ET, Sokoll LJ, Chan DW. Proteomic approaches to tumor marker discovery—identification of biomarkers for ovarian cancer. Arch Pathol Lab Med. 2002;126(12):1518–26.PubMedGoogle Scholar
- Zhang Z, Bast RC, Yu YH, Li JN, Sokoll LJ, Rai AJ, Rosenzweig JM, Cameron B, Wang YY, Meng XY, Berchuck A, van Haaften-Day C, Hacker NF, de Bruijn HWA, van der Zee AGJ, Jacobs IJ, Fung ET, Chan DW. Three biomarkers identified from serum proteomic analysis for the detection of early stage ovarian cancer. Can Res. 2004;64(16):5882–90.View ArticleGoogle Scholar
- Ueland FR, Desimone CP, Seamon LG, Miller RA, Goodrich S, Podzielinski I, Sokoll L, Smith A, van Nagell JR, Jr Zhang Z. Effectiveness of a multivariate index assay in the preoperative assessment of ovarian tumors. Obstet Gynecol. 2011;117(6):1289–97.View ArticlePubMedGoogle Scholar
- Cai G, Ma X, Zou W, Huang Y, Zhang J, Wang D, Chen B. Prediction value of intercellular adhesion molecule-1 gene polymorphisms for epithelial ovarian cancer risk, clinical features, and prognosis. Gene. 2014;546(1):117–23.View ArticlePubMedGoogle Scholar
- Köbel M, Madore J, Ramus SJ, Clarke BA, Pharoah PD, Deen S, Bowtell DD, Odunsi K, Menon U, Morrison C, Lele S, Bshara W, Sucheston L, Beckmann MW, Hein A, Thiel FC, Hartmann A, Wachter DL, Anglesio MS, Høgdall E, Jensen A, Høgdall C, Kalli KR, Fridley BL, Keeney GL, Fogarty ZC, Vierkant RA, Liu S, Cho S, Nelson G, Ghatage P, Gentry-Maharaj A, Gayther SA, Benjamin E, Widschwendter M, Intermaggio MP, Rosen B, Bernardini MQ, Mackay H, Oza A, Shaw P, Jimenez-Linan M, Driver KE, Alsop J, Mack M, Koziak JM, Steed H, Ewanowich C, DeFazio A, Chenevix-Trench G, Fereday S, Gao B, Johnatty SE, George J, Galletta L; AOCS Study Group, Goode EL, Kjær SK, Huntsman DG, Fasching PA, Moysich KB, Brenton JD, Kelemen LE. Evidence for a time-dependent association between FOLR1 expression and survival from ovarian carcinoma: implications for clinical testing. An Ovarian Tumour Tissue Analysis consortium study. Br J Cancer. 2014;111(12):2297–307.Google Scholar
- Luo XZ, He QZ, Wang K. Expression of Toll-like receptor 4 in ovarian serous adenocarcinoma and correlation with clinical stage and pathological grade. Int J Clin Exp Med. 2015;8(8):14323–7.PubMedPubMed CentralGoogle Scholar
- Ortolan E, Giacomino A, Martinetto F, Morone S, Lo Buono N, Ferrero E, Scagliotti G, Novello S, Orecchia S, Ruffini E, Rapa I, Righi L, Volante M, Funaro A. CD157 enhances malignant pleural mesothelioma aggressiveness and predicts poor clinical outcome. Oncotarget. 2014;5:6191–205.View ArticlePubMedPubMed CentralGoogle Scholar
- Morone S, Augeri S, Cuccioloni M, Mozzicafreddo M, Angeletti M, Lo Buono N, Giacomino A, Ortolan E, Funaro A. Binding of CD157 protein to fibronectin regulates cell adhesion and spreading. J Biol Chem. 2014;289(22):15588–601.View ArticlePubMedPubMed CentralGoogle Scholar
- Quarona V, Zaccarello G, Chillemi A, Brunetti E, Singh VK, Ferrero E, Funaro A, Horenstein AL, Malavasi F. CD38 and CD157: a long journey from activation markers to multifunctional molecules. Cytometry B Clin Cytom. 2013;84:207–17.View ArticlePubMedGoogle Scholar
- Morone S, Lo-Buono N, Parrotta R, Giacomino A, Nacci G, Brusco A, Larionov A, Ostano P, Mello-Grand M, Chiorino G, Ortolan E, Funaro A. Overexpression of CD157 contributes to epithelial ovarian cancer progression by promoting mesenchymal differentiation. PLoS ONE. 2012;7:e43649.View ArticlePubMedPubMed CentralGoogle Scholar
- Ortolan E, Arisio R, Morone S, Bovino P, Lo-Buono N, Nacci G, Parrotta R, Katsaros D, Rapa I, Migliaretti G, Ferrero E, Volante M, Funaro A. Functional role and prognostic significance of CD157 in ovarian carcinoma. J Natl Cancer Inst. 2010;102(15)1160–77.View ArticlePubMedGoogle Scholar