Fig. 3

Protein marker pre-qualification by mass spectrometry. A Potential prognostic biomarkers of prostate cancer were monitored in a prostatectomy cohort consisting of 38 patients with low-grade (NCCN 1, 2) and 40 patients with high-grade disease (NCCN 3, 4). Serum protein glycocapture was performed [15] and deamidated, formerly glycosylated peptides were monitored using PRM-MS. B From our list of 52 marker proteins, 33 were detected and quantified in our training cohort. The heatmap illustrates the intensity distribution of protein quantities over the cohort from ASPN (outside of the circle) to VTN (inside of the circle). Violin plots visualize data distribution and probability density. Distribution median and quartiles are shown in red. Single protein values are indicated by dots. Proteins that were used for machine learning are designated in green