- Open Access
Development of protein biomarkers in cerebrospinal fluid for secondary progressive multiple sclerosis using selected reaction monitoring mass spectrometry (SRM-MS)
© Jia et al.; licensee BioMed Central Ltd. 2012
- Received: 14 February 2012
- Accepted: 31 May 2012
- Published: 30 July 2012
Multiple sclerosis (MS) is a chronic inflammatory disorder of the central nervous system (CNS). It involves damage to the myelin sheath surrounding axons and to the axons themselves. MS most often presents with a series of relapses and remissions but then evolves over a variable period of time into a slowly progressive form of neurological dysfunction termed secondary progressive MS (SPMS). The reasons for this change in clinical presentation are unclear. The absence of a diagnostic marker means that there is a lag time of several years before the diagnosis of SPMS can be established. At the same time, understanding the mechanisms that underlie SPMS is critical to the development of rational therapies for this untreatable stage of the disease.
Using high performance liquid chromatography-coupled mass spectrometry (HPLC); we have established a highly specific and sensitive selected reaction monitoring (SRM) assay. Our multiplexed SRM assay has facilitated the simultaneous detection of surrogate peptides originating from 26 proteins present in cerebrospinal fluid (CSF). Protein levels in CSF were generally ~200-fold lower than that in human sera. A limit of detection (LOD) was determined to be as low as one femtomol. We processed and analysed CSF samples from a total of 22 patients with SPMS, 7 patients with SPMS treated with lamotrigine, 12 patients with non-inflammatory neurological disorders (NIND) and 10 healthy controls (HC) for the levels of these 26 selected potential protein biomarkers. Our SRM data found one protein showing significant difference between SPMS and HC, three proteins differing between SPMS and NIND, two proteins between NIND and HC, and 11 protein biomarkers showing significant difference between a lamotrigine-treated and untreated SPMS group. Principal component analysis (PCA) revealed that these 26 proteins were correlated, and could be represented by four principal components. Overall, we established an efficient platform to develop and verify protein biomarkers in CSF, which can be easily adapted to other proteins of interest related to neurodegenerative diseases.
A highly specific and sensitive multiplex SRM-MS assay was established for development and verification of CSF protein biomarkers in SPMS. Five proteins were found to be expressed significantly differently between the three cohorts, SPMS, NIND and HC and 11 proteins associated with lamotrigine treatment, which we expect will further our current understanding of SPMS disease pathology and/or therapeutic intervention.
- Multiple Sclerosis
- Select Reaction Monitoring
- Secondary Progressive Multiple Sclerosis
- Secondary Progressive Multiple Sclerosis
Multiple sclerosis (MS) is an inflammatory demyelinating disease of the CNS. During MS, myelin sheaths surrounding axons and the axons themselves are damaged as a result of chronic inflammation. Such inflammatory damage creates characteristic focal plaques in the white matter of the brain and spinal cord [1, 2]. Patients with MS initially present with a host of non-specific neurological symptoms and there is a high degree of variability in the early symptoms that patients experience. For almost all patients, however, early MS associated symptoms will occur as a series of relapses and remissions whereby a patient will remain almost entirely asymptomatic for an extended period of time between temporary symptom “relapses.” Over a variable period of time, MS will typically evolve from relapsing-remitting multiple sclerosis (RRMS) into a slowly progressive form of neurological dysfunction termed secondary progressive multiple sclerosis (SPMS) .
For a complex disease like MS, there is a good reason to believe that a change in the protein expression profile occurs long before clinical symptoms are established. Therefore, protein biomarkers differentially expressed in MS patients as compared to healthy individuals or patients suffering from other neurological disorders have great clinical potential - not only as early diagnostics, but as potential prognostic markers to be used for monitoring disease course and evaluating treatment efficacy [4–6]. Identification of such markers would also further our current understanding of MS disease pathology, pinpointing novel protein targets or signalling pathways for therapeutic intervention.
Mass spectrometry-based proteomic technologies have become preferred laboratory strategies for the discovery of diagnostic, prognostic, and therapeutic biomarkers [7, 8]. Since the early 1990s, a large number of potential protein biomarkers have been discovered every year in labs across the country - in both the academic and the industry sectors. However, only a handful of these identified targets are selected for further clinical investigation, fewer still have been formally validated, and of the thousands of markers that were identified between 2000 and 2005, only five new protein markers were approved by the US Food and Drug Administration (FDA) for measurement in sera or plasma in that time span . Indeed, since 1998, the introduction of new protein biomarkers approved by the US FDA has fallen to an average of one per year; and this trend continues to be true today despite intensified interest and investment from both academia and industry. While to some extent, these types of statistics reflect the inherent time-line needed to conduct discovery-based research, it has long been suggested that development of faster and more efficient biomarker verification strategies could greatly reduce the time and cost of biomarker-based diagnostic development and that the greatest bottleneck to the current biomarker pipeline occurs at the verification/validation stage , that is the stage at which purported biomarkers, obtained through any number of discovery strategies, are tested quantitatively on samples derived from a statistically significant number of subjects and controls.
There have been two commonly adopted strategies for obtaining these kinds of quantitative assays for protein biomarkers: the enzyme-linked immunosorbent assay (ELISA) utilizing antibodies to the proteins and mass spectrometry-based methods that target the predictable fragmentation of surrogate peptides. Immunoassays enjoy high specificity and high throughput; however, they depend critically on the availability of highly specific antibodies, the development of which is usually long and costly. In addition, there are technical limitations for multiplexing of immunoassays. In contrast, quantitative approaches using selected reaction monitoring (SRM) mass spectrometry, have recently drawn intense interest to protein biomarker verification/validation for advantages of enhanced sensitivity and specificity, ease of high throughput, and relatively low cost [8, 9, 11–15]. Using a tandem mass spectrometer in which the mass analysers act as two mass filters, one for the peptide molecular ion mass and the other for a known sequence fragment, SRM-MS provides a selectivity and specificity that in many cases enables one to simplify sample preparation by avoiding the initial immunoprecipitation or fractionation common in many other mass spectrometry-based strategies.
CSF has been a valuable diagnostic resource for neurodegenerative diseases because its composition directly reflects the metabolic process of the brain. Five proteins including interleukin (IL)-17 have been recently identified by Trojanowaski and colleagues as CSF biomarkers capable of differentiating between two forms of frontotemporal lobar degeneration . Similarly, amyloid beta (1–42), total tau and phosphorylated tau have been established as biomarkers for diagnosis of Alzheimer’s Disease (AD). Mattsson and colleagues found that combining biomarkers into a panel leads to a better predictive value than using individual biomarker for the diagnosis of AD . Interestingly, while the three AD biomarkers identified by Mattsson, et al.  had diagnostic value when patient CSF was interrogated, the proteins were either not detected in the peripheral blood or their measured concentration in plasma did not provide useful information for diagnosis. Given the results from the Mattsson study and how varied MS clinical presentation and disease progression can be across patients, it is highly unlikely that a single protein biomarker will be sufficient for a conclusive diagnosis of SPMS. It is equally unlikely that a single protein would define the pathophysiology of SPMS. It is far more probable that a biomarker dependent diagnosis will require a panel of disease indicators whereby different indicators will have greater or lesser diagnostic utility depending upon individual patients or stages of disease evolution. As such, our overall objective is to find a panel of CSF protein biomarkers that show differential expression profiles amongst SPMS patients, patients suffering from non-inflammatory neurological disorders, and disease-free controls. For the studies presented here, we also included subjects from the placebo-controlled clinical trial for lamotrigine as a putative neuroprotective therapy in secondary progressive MS from the UK. Though this clinical trial did not demonstrate a significant difference in clinical outcomes between patients taking lamotrigine (treated) and the placebo group (untreated) , we believed it was still beneficial to investigate the expression profiles of CSF protein biomarkers in subjects of this study to determine if the lamotrigine treatment has any observable effect at protein level. Based upon previous discovery results and an up-to-date literature survey [4, 19–21], we selected a total of 26 proteins as potential CSF protein biomarkers for SPMS and developed a mass spectrometric assay for relative quantitation.
Age, gender and diagnosis group for the 51 subjects
SPMS (treated) (2)
Limit of detection, retention time and peak area reproducibility
Differential expression profile of selected protein biomarkers in SPMS, NIND and healthy controls
Protein biomarkers and selected principal components with significant difference between three groups ( p <0.01)
SPMS vs. HC
SPMS vs. NIND
NIND vs. HC
Differential expression profile of selected protein biomarkers in lamotrigine trial
Protein biomarkers with significant difference ( p <0.01) between lamotrigine treated and untreated SPMS group
Principal component analysis
Protein biomarker variable loadings on the four rotated principal components
By taking the advantage of high specificity and sensitivity, we have developed a multiplex SRM assay for detecting even low abundant proteins in CSF with relatively simple sample preparation. The platform we have developed here can be easily adapted to other protein biomarker studies. The sensitivity and throughput might be further enhanced using newly developed technologies, such as the dual stage electrodynamic ion funnel interface described by Hossain et al. . Although our assay successfully quantified both high abundant and low abundant proteins in CSF, several proteins which have been previously reported present in CSF were not detected during our initial method development using pooled CSF, such as nidogen-2 (NID2) and nitric oxide synthase (NOS2). Low abundance might be one of the contributing factors, though the intrinsic biological properties of the pooled CSF and the instability of peptides/proteins are also possible. Improvements in sample preparation and optimization of instrument parameters could increase the appearance of low abundant proteins in future studies.
The human proteome is more complex, compared to the genome, considering that each protein can be present in different isoforms even at the same time. However, mass spectrometry-based targeted proteomics has the advantage of specifically quantifying individual protein isoform, which could potentially help us better understand the pathogenesis underlying various diseases. Although we did not include such studies here, the intrinsic specificity of SRM provides the merit to do so if necessary in the future.
Despite CSF’s unique value for neurodegenerative diseases, there were some concerns in the process of searching for protein biomarkers in CSF, such as low protein abundance, difficulties in collecting samples, spontaneous variation through the day, etc. It is essential that CSF collections follow standard operating procedures, including but not limited to the following: all lumbar punctures were carried out at approximately the same time of day, and sample processing (e.g. time to centrifugation, centrifugation speed and time) and storage was handled in a standard way. Our study was carried out among a limited number of patients since the purpose of our study was to validate the feasibility of using SRM for protein biomarker verification. In the future, validation studies on large cohorts of samples originating from healthy controls, relapse-remitting MS (RRMS), SPMS, primary-progressive MS (PPMS), NIND and other inflammatory neurological disease (OIND) controls will determine clinical value of this pilot combinatorial biomarker.
Using SRM to target a specific set of surrogate biomarker peptides, we established a highly sensitive and specific multiplex assay to simultaneously detect twenty-eight potential protein biomarkers which might be involved in the pathology of SPMS. We quantified the relative levels of each targeted protein using an internal standard horse apomyoglobin and compared their expression profiles among three different cohorts: SPMS, NINDS and healthy controls. ANOVA indicated one protein biomarker- showing significantly different expression between SPMS and HC, and three protein biomarkers showing significantly different expression between SPMS and NIND. It is highly possible that these four proteins play a role in the pathophysiology of SPMS and could potentially benefit the diagnosis, prognosis and/or the development of newer generation treatments. In addition, two proteins were expressed differently between NIND and healthy controls, which could potentially benefit NIND studies. In the lamotrigine trial, although the trial itself came negative with respect to the efficacy of the drug therapy, our analysis suggested that eleven proteins in the treated group had significantly lower expression compared to the untreated group. Since lamotrigine therapy had an effect of decreasing expression for these proteins, it is plausible that these proteins are correlated with other proteins. In fact, our principal component analysis (PCA) revealed that these 26 proteins were correlated, and could be represented by four principal components.Overall, we have established a straightforward mass spectrometry-based platform for CSF protein biomarker development and verification. This platform can be easily adapted to study a broad range of proteins, especially feasible for those of interest to neurodegenerative diseases.
Subjects and clinical samples
CSF samples involved in our study included a coded cohort of 12 untreated SPMS patients and untreated 12 non-inflammatory neurological disorders (NIND) controls from the Neuroimmunology Branch of the National Institutes of Neurological Disorders and Stroke (NIB/NINDS/NIH) collected under natural history protocol 09-N-0032, healthy controls from University of Hawaii, and SPMS patients from a UK double blinded lamotrigine trial (active arm and placebo). All patients signed informed consent and all research procedures were approved by the institutional review boards (IRB) of afore-mentioned institutions.
Cerebrospinal fluid sample preparation
NIB samples were transported to the laboratory on ice and spun (3000 g x 10 min) within 30 min of collection. Cell-free supernatants were sequentially coded and immediately cryopreserved at −80°C in 500 μl aliquots.
Prior to all sample preparation, we prepared a stock of 1pmol/μL of horse apomyoglobin in deionized water to be used as an internal standard spiked into all CSF samples. Prior to tryptic digestion of the CSF samples used both in method development and then in our cohort screening experiments, 100 μL of 0.1% Rapigest (Waters, Milford, MA) resuspended in 100 mM ammonium bicarbonate, 2 μL 500 mM dithiothreitol (DTT), and 10 μL of the 1pmol/μL stock of the internal standard, horse apomyoglobin, were added to each individual 100 μL CSF aliquot. To reduce protein disulfide bonding, each sample aliquot was heated at 60°C for 30 min. After allowing each sample cool down to room temperature, 10 μL of 200 mM iodoacetamide (IA) was added. Each vial was placed in the dark for 30 min to allow for alkylation of all free protein cysteine residues. 5 μL of trypsin (0.2 μg/μL in 1 mM HCl) was then added to each sample following the DTT reduction and IA alkylation steps. The sample mixtures were incubated at 37°C for 18 h to allow for complete enzymatic digestion of the CSF and the protein standard horse apomyoglobin. Following trypsinization, 2 μL of trifluoroacetic acid was added to the digestion mixture to quench the reaction. The samples were then incubated at 37°C for an additional 45 min. Following this second incubation step, samples were speed-vacuumed to dryness, reconstituted in 0.1% formic acid in deionized water, and desalted using Waters Oasis HLB solid phase extraction cartridges (Waters, Milford, MA) according to the manufacturer’s protocol with a vacuum manifold. To prepare for LC-MS analysis, the eluents from the solid phase extraction were speed-vacuumed to dryness and reconstituted with 20 μL 0.1% formic acid in water. Pooled CSF samples obtained from patients without identified medical records were used during the initial stages of method development. All reagents were added to practice CSF in proportion as described. Diseased CSF and healthy control CSF samples selected for inclusion for our cohort screening experiments were obtained from patients with complete associated medical records. Individual CSF patient samples used during cohort screening were blinded prior to any sample processing. Additionally, sample aliquots were prepared in parallel and as duplicates to minimize experimental error during sample preparation. All reagents were purchased from Sigma if not mentioned specifically.
Prior to our selected reaction monitoring assay development efforts, the RP-HPLC gradient profile used for separation of digested CSF samples was first optimized. An Agilent ZORBAX SB-C18 column (150 X 0.5 mm, 5 μm) (Agilent Technologies Inc., Santa Clara, CA, USA) was attached to a Waters Nano-Acquity ultra-high pressure HPLC (Waters, Milford, MA, USA) and placed in the front end of our Thermo Vantage Triple Quadrupole mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Total Ion Chromatograms of the various c18 RP-HPLC separations were acquired in full scan mode acquisition with a scan time of 0.5 s. Ultimately, we selected a 32 min RP-HPLC linear gradient to be used for all subsequent CSF sample separation whereby the injected peptides were eluted with the following gradient: the first 2.4 min post-injection were diverted to waste with a 3% B hold, a linear 3-45% B gradient was performed in the next 22.6 min, a linear 45-90% B gradient ramp was performed in 1 min followed by a 90-100% B linear ramp in 1 min, 100% B was held for 1 min before a linear gradient of 100-3% B was performed in 2 min followed finally by a 3% B hold for 2 min. Solvent A consisted of 0.1% formic acid in water and solvent B consisted of 90% acetonitrile, 10% water and 0.1% formic acid. The flow rate of the Nano-Acquity HPLC was set at 20 μL/min.
Intact horse apomyoglobin was chosen as a model protein to establish the LOD of our multiplex SRM LC-MS/MS method. Digested horse apomyoglobin was diluted in serial in digested CSF with concentrations ranging from 1 fmol/μL to 100 fmol/μL. Using a CSF sample with 100 fmol/μL of digested horse apomyoglobin, the following was experimentally determined: the signature tryptic peptide NDIAAK was selected as the surrogate peptide for the apomyoglobin protein, the three most abundant detected transitions of peptide NDIAAK, ions y3, y4, and y5 were selected for detection in Q3, and of those three, the most abundant, y3, was selected for generating a standard curve for this peptide. A serial of apomyoglobin dilutions were then subject to LC-MS/MS analysis with Q1 mass filter targeting detection of the parent ion of NDIAAK and Q3 mass filter targeting detection of the three selected product ions. Ions were introduced into the mass spectrometer via an H-ESI II probe outfitted with a 32 gauge needle (Thermo Fisher Scientific, Waltham, MA, USA). Instrument operating parameters used during acquisition were as follows: capillary temperature of 270°C, vaporizing heat was shut off, sheath gas pressure was set at 10, no auxiliary gas was used, and the spray voltage was set to 4,000 V. Instrument settings in Xcaliber were as follows: positive scan mode, scan widths of 0.004 m/z, scan times of 0.015 s, chrom filters enabled and set at 50, collision gas set at 1.5mTorr, Q1 peak width (FWHM) of 0.7, Q3 peak width of (FWHM) 0.7, and cycle times of 5 s (Xcaliber v 2.1, Thermo Fisher Scientific, Waltham, MA, USA). Following data acquisition, peak areas under the curve were calculated using an open source proteomic software platform Skyline  (Skyline v. 1.1, MacCoss Lab, Seattle, WA) and a linear standard curve (y = 5884.7x-9111.7, R2 = 0.9998) was generated using Microsoft Excel (Figure 1).
LC-MS/MS method development and optimization
Using a Waters ultra-high pressure reverse phase high performance liquid chromatography (Waters nanoACQUITY UPLC, Milford, MA, USA) outfitted with an Agilent ZORBAX SB-C18 column (150 X 0.5 mm, 5 μm) (Agilent Technologies Inc., Santa Clara, CA, USA) and coupled to a Thermo Fisher triple quadrupole mass spectrometer (Thermo TSQ Vantage, Thermo Fisher Scientific, Waltham, MA, USA), we designed a multiplexed peptide based selected reaction monitoring (SRM) assay using the RP-HPLC and mass spectrometric settings mentioned in the two previous method sections. Our SRM assay allows for the simultaneous relative quantification of 26 tryptic peptides originating from proteins implicated in SPMS pathology. FASTA files for selected protein biomarkers were downloaded from Uniprot (http://www.uniprot.org) individually and imported into Skyline. The uniqueness of the selected surrogate peptides was confirmed by running individual BLAST search from NCBI website (http://blast.ncbi.nlm.nih.gov/Blast.cgi). Instrumental methods were exported from Skyline into a Thermo Xcaliber 2.1 “EZ SRM” method template with the same settings as used during data acquisition for our LOD determination experiments (Xcaliber v. 2.1, Thermo Fisher Scientific, Milford, MA, USA). To design our SRM assay, we adopted an iterative method development strategy, eliminating poor performing transitions from the SRM assay in a targeted method refinement cycle . Raw data was inspected in the Skyline software platform and the peak areas of each detected transition were normalized to that of horse apomyoglobin peptide HGTVVLTALGGILK. Starting with an initial 1885 transitions for 403 surrogate peptides, we completed seven rounds of iterative method refinement to generate the final SRM assay. The final SRM assay was a scheduled method consisting of 78 transitions for 26 peptides with a scheduling window of four minutes - no more than 50 concurrent transitions were detected at any given time. Reproducibility was confirmed by running four replicates. Both retention time and peak area are highly reproducible (Figure 3a and 3b).
Box-Cox transformation was applied to CSF values of the 26 biomarkers. Age and gender were considered as covariates, p-value of 0.1 was used for covariate selection. Analysis of variance (ANOVA) with or without covariates was performed to assess the differences in the CFS biomarkers values between three cohorts (22 SPMS, 12 NIND and 10 HC), followed by Scheffe’s pair-wise comparison of means. ANOVA was also applied to assess the effect of lamotrigine therapy on the expression of 26 protein biomarkers by comparing 22 SPMS patients with 7 lamotrigine treated SPMS patients.
The relationship between the 26 biomarkers was estimated by Pearson correlation coefficients. Since these biomarkers were found to be closely correlated, principal component analysis (PCA) with Varimax rotation was performed to represent the 26 biomarkers as a set of new orthogonal variables, and subsequently, ANOVA with or without covariates was applied to the selected principal components.
A significant level of 0.01 was used in order to adjust for multiple testing. The statistical analyses were performed using SAS version 9.2.
This study was supported by a generous grant from Robert Snyder. Mass spectrometry was carried out at the Middle Atlantic Mass Spectrometry Laboratory at the Johns Hopkins University School of Medicine. RJC, YJ and CJ were supported in part by a grant R01NS039253 (Cotter) from the National Institute of Neurological Disease and Stroke and a contract HHSN268201000032C (VanEyk) from the National Heart Lung and Blood Institute. Measurements were obtained on a triple quadrupole mass spectrometer purchased through a grant 1S10RR025419-01 (Cotter RJ) from the Shared Instrument Grant (SIG) Program of the National Institutes of Health Division of Research Resources. Funding for the lamotrigine trial was obtained from the UK MS Society and NMSS. Collection of NINDS samples was supported by the intramural research program of NINDS/NIH.
- Bruck W: The pathology of multiple sclerosis is the result of focal inflammatory demyelination with axonal damage. J Neurology. 2005, 252 (Supplement 5): v3-v9.View ArticleGoogle Scholar
- Confavreux C, Vukusic S: Multiple sclerosis: a degenerative disease?. Bull Acad Natl Med. 2008, 192: 483-491.PubMedGoogle Scholar
- Rovaris M, Confavreux C, Furlan R, Kappos L, Comi G, Filippi M: Secondary progressive multiple sclerosis: current knowledge and future challenges. Lancet Neurol. 2006, 5: 343-354. 10.1016/S1474-4422(06)70410-0View ArticlePubMedGoogle Scholar
- Harris V, Sadig SA: Disease biomarkers in multiple sclerosis: Potential for use in therapeutic decision making. Mol Diagn Ther. 2009, 13: 225-244. 10.1007/BF03256329View ArticlePubMedGoogle Scholar
- Hammack BN, Fung KY, Hunsucker SW, Duncan MW, Burgoon MP, Ownes GP, Gilden DH: Proteomic analysis of multiple sclerosis cerebral spinal fluid. Mult Scler. 2004, 10: 245-260. 10.1191/1352458504ms1023oaView ArticlePubMedGoogle Scholar
- Dumont D, Noben J-P, Raus J, Stinissen P, Robben J: Proteomic analysis of cerebrospinal fluid from multiple sclerosis patients. Proteomics. 2004, 4: 2117-2124. 10.1002/pmic.200300715View ArticlePubMedGoogle Scholar
- Hortin GL: Can Mass Spectrometric Protein Profiling Meet Desired Standards of Clinical Laboratory Practice?. Clin Chem. 2005, 51: 3-5.View ArticlePubMedGoogle Scholar
- Lopez MF, Rezai T, Sarracino DA, Prakash A, Krastins B, Athanas M, Singh RJ, Barnidge DR, Oran P, Borges C, Nelson RW: Selected Reaction Monitoring–Mass Spectrometric Immunoassay Responsive to Parathyroid Hormone and Related Variants. Clin Chem. 2010, 56: 281-290. 10.1373/clinchem.2009.137323View ArticlePubMedGoogle Scholar
- Carr SA, Anderson L: Protein Quantitation through Targeted Mass Spectrometry: The Way Out of Biomarker Purgatory?. Clin Chem. 2008, 54: 1749-1752. 10.1373/clinchem.2008.114686PubMed CentralView ArticlePubMedGoogle Scholar
- Anderson L: The Roles of Multiple Proteomic Platforms in a Pipeline for New Diagnostics. Mol Cell Proteomics. 2005, 4: 1441-1444. 10.1074/mcp.I500001-MCP200View ArticlePubMedGoogle Scholar
- Picotti P, Rinner O, Stallmach R, Dautel F, Farrah T, Domon B, Wenschuh H, Aebersold R: High-throughput generation of selected reaction-monitoring assays for proteins and proteomes. Nat Methods. 2010, 7: 43-46. 10.1038/nmeth.1408View ArticlePubMedGoogle Scholar
- Prakash A, Tomazela DA, Frewen B, MacLean B, Merrihew G, Peterman S, MacCoss MJ: Expediting the development of targeted SRM assays: using data from shotgn proteomics to automate method development. J Proteome Res. 2010, 9: 6678-6688. 10.1021/pr100821mView ArticlePubMedGoogle Scholar
- Addona TA, Abbatiello SE, Schilling B, Skates SJ, Mani DR, Bunk DM, Spiegelman CH, Zimmerman LJ, Ham AJL, Keshishian H, Hall SC, Allen S, Blackman RK, Borchers CH, Buck C, Cardasis HL, Cusack MP, Dodder NG, Gibson BW, Held JM, Hiltke T, Jackson A, Johansen EB, Kinsinger CR, Li J, Mesri M, Neubert TA, Nile RK, Pulsipher TC, Ransohoff D, Rodriguez H, Rudnick PA, Smith D, Tabb DL, Tegeler TJ, Variyath AM, Vega-Montoto LJ, Wahlander A, Waldemarson S, Wang M, Whiteaker JR, Zhao L, Anderson NL, Fisher SJ, Liebler DC, Paulovich AG, Regnier FE, Tempst P, Carr SA: Multi-site Assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma. Nat Biotechnol. 2009, 27: 633-641. 10.1038/nbt.1546PubMed CentralView ArticlePubMedGoogle Scholar
- Keshishian H, Addona T, Burgess M, Mani DR, Shi X, Kuhn E, Sabatine MS, Gerszten RE, Carr SA: Quantification of Cardiovascular Biomarkers in Patient Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution. Mol Cell Proteomics. 2009, 8: 2339-2349. 10.1074/mcp.M900140-MCP200PubMed CentralView ArticlePubMedGoogle Scholar
- Nilsson T, Mann M, Aebersold R, Yates JR, Bairoch A, Bergeron JJM: Mass spectrometry in high-throughput proteomics: ready for the big time. Nat Methods. 2010, 7: 681-685. 10.1038/nmeth0910-681View ArticlePubMedGoogle Scholar
- Jones N: Neurodegenerative disease: CSF biomarkers differentiate between two forms of frontotemporal lobar degeneration. Nat Rev Neurol. 2011, 7: 4-View ArticlePubMedGoogle Scholar
- Mattsson N, Zetterberg H, Hansson O, Andreasen N, Parnetti L, Jonsson M, Herukka S-K, van der Flier WM, Blankenstein MA, Ewers M, Rich K, Kaiser E, Verbeek M, Tsolaki M, Mulugeta E, Rosén E, Aarsland D, Visser PJ, Schröder J, Marcusson J, de Leon M, Hampel H, Scheltens P, Pirttilä T, Wallin A, Eriksdotter Jönhagen M, Minthon L, Winblad B, Blennow K: CSF Biomarkers and Incipient Alzheimer Disease in Patients With Mild Cognitive Impairment. JAMA. 2009, 302: 385-393. 10.1001/jama.2009.1064View ArticlePubMedGoogle Scholar
- Kapoor R, Furby J, Hayton T, Smith KJ, Altmann DR, Brenner R, Chataway J, Hughes RAC, Miller DH: Lamotrigine for neuroprotection in secondary progressive multiple sclerosis: a randomised, double-blind, placebo-controlled, parallel-group trial. Lancet Neurol. 2010, 9 (7): 681-688. 10.1016/S1474-4422(10)70131-9View ArticlePubMedGoogle Scholar
- Salter H: WO/2010/005387,Google Scholar
- Scarisbrick IA, Linbo R, Vandell AG, Keegan M, Blaber SI, Blaber M, Sneve D, Lucchinetti CL, Rodriguez M, Diamandis EP: Kallikreins are associated with secondary progressive multiple sclerosis and promote neurodegeneration. Biol Chem. 2008, 389: 739-745.PubMed CentralView ArticlePubMedGoogle Scholar
- Satoh J-I, Yukitake M, Kurohara K, Takashima H, Kuroda Y: Detection of the 14-3-3 protein in the cerebrospinal fluid of Japanese multiple sclerosis patients presenting with severe myelitis. J Neuorological Sciences. 2003, 212: 11-20. 10.1016/S0022-510X(03)00083-2. 10.1016/S0022-510X(03)00083-2View ArticleGoogle Scholar
- Hossain M, Kaleta DT, Robinson EW, Liu T, Zhao R, Page JS, Kelly RT, Moore RJ, Tang K, Camp DG, Qian W-J, Smith RD: Enhanced Sensitivity for Selected Reaction Monitoring Mass Spectrometry-based Targeted Proteomics Using a Dual Stage Electrodynamic Ion Funnel Interface. Mol Cell Proteomics. 2011, 10: doi:10.1074/mcp.M000062-MCP201.Google Scholar
- MacLean B, Tomazela DM, Shulman N, Chambers M, Finney GL, Frewen B, Kern R, Tabb DL, Liebler DC, MacCoss MJ: Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics. 2010, 26: 966-968. 10.1093/bioinformatics/btq054PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.